before starting:

reminders:

tutorial!!!!!!!!!

• dr expects us to do it seriously, as if we’re sitting in an exam

bit of change in lecture series

should be 2 things covered today

1. spectrometer and mass spectrometer
2. instead changed to this + spectrophotometry

until lecture 11 is ica

review of last week

• this particular lecture covers HPLC, instrument commonly used for chromatogrpaphy
  • all sort of diagnostics

integration … → when you have a mixture of proteins

e.g a question → mixture protein in tutorial, how would you design a method that purify protein single or multiple, or all in the mixture?

HPLC

we’ll see which of the 3 methods is the suitable for protein

or if it’s not single, which combination would be suitable?