similar to immunoassays

• when you run the gel, the structure of it is gel
  • it’s fragile
  • blotting is
    • transfer whatever is in that gel (make a copy) in another media which is a nitroceclouous membrane or PEBF?

run electrophoresis

• copywhatever in that gel into the nitrocolceus paper

as they stick in paper, we can further manipulat to detect the molecule of interest

1. transfer of separated nucleic acids (from electrophoresis) into membrane
   1. blotting is done AFTER electrophoreiss
2. detection of taarget molecule:
   1. the one we’re interested it
      1. e.g it’s an antigen
      2. we react to an antibody specific to it, we’ll see that protein of interest

western → protein analysis (uses antibody)

southern → DNA

northern → RNA

southern was the first for DNA, then the names were adapted for RNA and proten