objectives

why are there many types of PCR?

• intended use
• technique
• target?

due to PCR limitations

1. polymerase activity:
   1. how long it can polyemerise
2. yield (quality and quantity)
   1. sometimes not specific
3. nature\source of the template:
   1. RT transcripatee comes by and also in situ
4. unknown DNA sequence
   1. inverse PCR

before that, “revision”

make billions of copies in 2 hours

what do we know about DNA polymerase?