we missed last week’s lab

• schedule doesnt have any empty spot to move the lab forwards

last lecture, we mentioned the fact

• although the first few lectures were general things we already had been introduced to:
  • the next stuff are biochemical techniques we dont know much of

references!

• make sure to check them
  • read, comprehend, examine them
    • these are references for 2 reasons:
      • whatever we present as bulletpoints\figures dont have all the details
        • even though we mentioned the details, you may find during those lectures, the details are not clear or good eneough → go check the references for answers
• most of this lecture is coming from the first REFERENCE
• 2nd ref is a technical catalogue

the tools we use are sold and described by the manufacturer, the best information then is from the catalogue

forward path from today to almost the last lecture we’ll have

• the purpose of this codemap?
  • illustrate one important thing -. in biomedical…. sometimes we become interested in a certain molecule (most of the time proteins)
    • that protein must have a source

      • that source could be a tissue (e.g you have an interest in an enzyme which is a liver enzyme)

      • in some cases, the source, you may have cells of the tissue available either by having them borrorwed from somewhere, or from some case, purchased

      • these tissues have the protein of interest

      • eventually hwat we want to have is → to know if this protein has a function [predict the function of protein]

        • characterize the size of the prtein → electrophoresis
        • define the amount of protein purified → spectrophotomercy
    • in order to reach the final application, steps:
  1. how do we harvest the cell or cell content(whatever inside the cells)
     1. we get a homogenate (contains soluble and insoluble molecules)
  2. once we harvest in 1 way or another, we separate the soluble from insoluble
     1. centrifugation
  3. whatever is in the supernatant contains many many different components
     1. proteins, etc
     2. we have to find a way to purify that protein (chromatography)
  4. once you have the protein purified
     1. check if purified via characterization
        1. measure concentration
        2. size
        3. function
        4. etc

preparation of a homogenate (harvest a protein from a tissue)

to get a protein out of a tissue, we must first prepare a homogenate