we already get into why we centrifugate

we are interested to characterize a protein

in the initial stage, you have a tissue\cell culture\whatever

you homogenize

we alos learned that we want to remove some solid material using a filter (gauze)

eventually we end up with a mixture of things (particles)

in order to remove the particles from the solvent (eventually caleld supernanatant)

we need a tecnnique to allow us to sedement the particles eiether completely in one go or multiple steps

• one go → remvoe any particle in the mixture
  • sometimes we might need to do “fractioning” → removal of various particles step-wise

centrifugation

allows the paraticles to be sedemented in 1 go or multiple

eventually we end up with a supernatant (soluble material; usually aqeouos material) and pellet (insoluble particles)

separataion by sedementation