today

• Digital pcr is the third gen of PCR

In qpcr, a reference is needed when wanting to knwo dna yield

Do qpcr, and have reference sample → tell you the dilutions, we know concentration of the dilution, then get standard curve to get the conc of the tested sample

All of. This is not needed in pc

We go a the level of molecular quantification

Every single piece of sna in that test sample is quantifed

Partition → make sure you separate small quantities of the dna sample into small chambers

Im a fna sample you want to quantify

Mix with reagent to wuantify (e.g fluorescent