how do you make sure the DNA \ RNA you extracted is good?
- couple of tests we can use
we’ll do 2 of them
nano-drop\
gel-electrophoresis
why? we need to make sure we have a good pure dna \ rna sample?
- downstream experiments
- no point in me doing an experiment, and then waste all of it because the dna is not good
- test to make sure we proceed further
the technique measure nucleic acid in a solution or within a gel-matrix
- uv absorbance (similar to spectrometry)
- anything in life can do things when exposed to light:
1.
- fluoroscense staining