Reception:

rejection criteria:

1. QNS “quantity not sufficient” (special condition for sodium citrate tubes to achieve the 1:9 ratio and avoid false results)
2. Hemolyzed → plasma is red ish
3. lipemic plasma (turbid)
4. blood collected in a syringe
5. urine collected in stool
6. mismatch in label / unlabeled
7. leakage
   1. form contamination from leakage
8. plasma itself is contaminated
9. correct tube

blood film:

• you want to achieve:
  • tongue shape
  • only 2/3rd of the slide smeared
  • not thin, but also blood not touching the borders

Sickledex:

need:

1. whole blood
2. negative and positive control
3. prepared reagent

procedure:

1. add 2mL of reagent on each tube
2. add 20 uL of (controls a+ sample0
3. mix using droppers/pipettes
4. wait for 5 minutes

principle of test is HbS solubility

positive test → presence of HbS which is insoluble in this case → turbid and lines not covered