National Center for Biotechnology Information (nih.gov)

Ensembl genome browser 111

Mutalyzer 3

SNPCheck (genetools.org)

UCSC In-Silico PCR

today’s topic is [title]

there is am utation and we want to detect it

what are the methods?

1. PCR
2. RFLP

PCR

denaturation

annealing

• we need primer \ olignonucleotides which bind
  • we have to design them
  • we’ll learn how to design them

extension