tube aggluation test

• serial dilution
  • put the antigen on the sample (blood) suspected to have antibodies

ELISA

underoges multiple steps

• put the antibodies on the antigens present in the wells

Substrate binds with enzyme to produce color

RPR

• used for non-tropenomal antibody (produced by cells infeced) →these are called reagant (the reagant used with the RPR test)

we then confirm it further with TPHA